D., Senior Scientist, Oncology i Med, Astra Zeneca Preclinical in vitro models that better represent the complexity of an in situ human tumor are being sought as part of the IMI project PREDECT (
As part of PREDECT we have been investigating the use of organotypic slice culture for cultivation of tumor material ex vivo.
High-Throughput Screening Using a Primary Human 3D Organotypic Culture Hilary Kenny, Ph.
Coffee Break in the Exhibit Hall with Poster Viewing Addressing Challenges in Organoid Culture Using a High-Throughput Microraft Array Platform Adam D. D., Postdoctoral Fellow, Magness Lab, Gastroenterology and Hepatology, University of North Carolina, Chapel Hill Organoid cultures have emerged as a powerful tool for understanding adult stem cell biology in a wide range of tissues.
However, conventional organoid methodology presents unique challenges in terms of accurate quantification and retrieval of samples.
D., Research Associate (Assistant Professor), Obstetrics & Gynecology, University of Chicago The tumor microenvironment contributes to cancer progression, metastasis and drug resistance.
A multilayered culture containing primary human fibroblasts, mesothelial cells and extracellular matrix was adapted into a robust and reliable 384- and 1,536-multi-well high-throughput screening assay that reproduces the human ovarian cancer metastatic microenvironment.
Collectively, these findings show that a 3D organotypic culture can be adapted for high-throughput screening.
Comparing 2D Kidney Cell and 3D Kidney Organoid Cultures for in vitro Drug Toxicity Assays David Grainger, Ph.
One of our clients, Janssen Incubator, developed a natural product drug discovery platform using natural product scaffold-based chemical entities.